preliminary results of treating cancerous cells of lung (qu-db) by hyperthermia using diode laser and gold coated fe3o4/sio2 nano-shells: an in-vitro assay

introduction in this study, we describe the results of controlled synthesis and application of gold coated fe3o4/sio2 nano-shells combined with the optical property of gold for enhancement of selective photothermal interaction with cancerous cells based on the surface plasmon resonance (spr) phenomena. materials and methods magnetite nano-particles (mnps) were prepared by means of co-precipitation. mnps were modified with a thin layer of silica using the stober method. the amino-modified fe3o4/sio2 nano-shells were covered with gold colloids as a self-assembeled process. in-vitro assays were performed to determine the effect of apoptosis of the cells based on the cells morphological changes. results the biologically inert nano-shells (85 nm) with a magnetite/silica core and a gold shell were optically activated. a successful laser-hyperthermia based on the thermal effect of surface plasmon resonance was performed using different gold concentrations. the thermal profile effects of laser power are presented as ideal cases of nanoshell-assisted photo-thermal therapy. the thermally-induced cell death has been shown to be dependent on nps concentration and laser power density. the power densities of 157 and 184 w/cm2 caused complete cell death at the focal point of the laser beam.cell damage was reduced by decreasing the power density of laser. also, a larger area of damage on cell culture plates was observed at longer intervals of laser irradiation. conclusion an optimized laser-(spr) hyperthermia was obtained using a concentration of gold coated fe3o4/sio2 nano-shells concentration=0.1 mg/ml at intensity=157 w/cm2 at 60s.